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osteogenic induction media  (Thermo Fisher)


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    Thermo Fisher osteogenic induction media
    Osteogenic Induction Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteogenic+induction+media/pm40346614-61-12-15?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    osteogenic induction media - by Bioz Stars, 2026-07
    90/100 stars

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    The DDM extract promotes Treg cells differentiation via IDO1 to enhance BMSCs viability and <t>osteogenic</t> differentiation in the co-culture system of naïve CD4 + T cells and BMSCs. A, CCK-8 results showed that the DDM extract significantly increased BMSCs viability, which decreased with the IDO1 inhibitor. No significant difference was observed between the Control and DDM+Inhibitor groups. B, Azide-Azide coupling method for ALP staining, with the red arrow indicating the blue precipitate after staining. ALP content significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. C, ARS staining, with the white arrow indicating the red calcium nodules after staining. Calcium nodules significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. WB (D) and RT-qPCR (E) results indicated that the DDM extract significantly upregulated RUNX2, BSP, Osx, COL I, OPN, OCN, OPG and RANKL. With the DDM+IDO1 inhibitor, RUNX2, BSP, Osx, COL I, OPN, OCN and OPG decreased significantly while RANKL remained unchanged. Neither DDM nor DDM+inhibitor affected RANK expression. CD4+BMSC, co-culture of naïve CD4 + T cells and BMSCs; DDM+BMSC, culture of BMSCs with the DDM extract; CD4+DDM+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract; CD4+DDM+Inh+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract and IDO1 inhibitor; DDM+Inh+BMSC, culture of BMSCs with the DDM extract and IDO1 inhibitor.
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    The DDM extract promotes Treg cells differentiation via IDO1 to enhance BMSCs viability and <t>osteogenic</t> differentiation in the co-culture system of naïve CD4 + T cells and BMSCs. A, CCK-8 results showed that the DDM extract significantly increased BMSCs viability, which decreased with the IDO1 inhibitor. No significant difference was observed between the Control and DDM+Inhibitor groups. B, Azide-Azide coupling method for ALP staining, with the red arrow indicating the blue precipitate after staining. ALP content significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. C, ARS staining, with the white arrow indicating the red calcium nodules after staining. Calcium nodules significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. WB (D) and RT-qPCR (E) results indicated that the DDM extract significantly upregulated RUNX2, BSP, Osx, COL I, OPN, OCN, OPG and RANKL. With the DDM+IDO1 inhibitor, RUNX2, BSP, Osx, COL I, OPN, OCN and OPG decreased significantly while RANKL remained unchanged. Neither DDM nor DDM+inhibitor affected RANK expression. CD4+BMSC, co-culture of naïve CD4 + T cells and BMSCs; DDM+BMSC, culture of BMSCs with the DDM extract; CD4+DDM+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract; CD4+DDM+Inh+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract and IDO1 inhibitor; DDM+Inh+BMSC, culture of BMSCs with the DDM extract and IDO1 inhibitor.
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    The DDM extract promotes Treg cells differentiation via IDO1 to enhance BMSCs viability and osteogenic differentiation in the co-culture system of naïve CD4 + T cells and BMSCs. A, CCK-8 results showed that the DDM extract significantly increased BMSCs viability, which decreased with the IDO1 inhibitor. No significant difference was observed between the Control and DDM+Inhibitor groups. B, Azide-Azide coupling method for ALP staining, with the red arrow indicating the blue precipitate after staining. ALP content significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. C, ARS staining, with the white arrow indicating the red calcium nodules after staining. Calcium nodules significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. WB (D) and RT-qPCR (E) results indicated that the DDM extract significantly upregulated RUNX2, BSP, Osx, COL I, OPN, OCN, OPG and RANKL. With the DDM+IDO1 inhibitor, RUNX2, BSP, Osx, COL I, OPN, OCN and OPG decreased significantly while RANKL remained unchanged. Neither DDM nor DDM+inhibitor affected RANK expression. CD4+BMSC, co-culture of naïve CD4 + T cells and BMSCs; DDM+BMSC, culture of BMSCs with the DDM extract; CD4+DDM+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract; CD4+DDM+Inh+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract and IDO1 inhibitor; DDM+Inh+BMSC, culture of BMSCs with the DDM extract and IDO1 inhibitor.

    Journal: International Dental Journal

    Article Title: Demineralized Dentin Matrix Promotes Bone Regeneration Through IDO1-Mediated Th17/Treg Cell Balance Modulation

    doi: 10.1016/j.identj.2025.103853

    Figure Lengend Snippet: The DDM extract promotes Treg cells differentiation via IDO1 to enhance BMSCs viability and osteogenic differentiation in the co-culture system of naïve CD4 + T cells and BMSCs. A, CCK-8 results showed that the DDM extract significantly increased BMSCs viability, which decreased with the IDO1 inhibitor. No significant difference was observed between the Control and DDM+Inhibitor groups. B, Azide-Azide coupling method for ALP staining, with the red arrow indicating the blue precipitate after staining. ALP content significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. C, ARS staining, with the white arrow indicating the red calcium nodules after staining. Calcium nodules significantly increased with the DDM extract but decreased with the DDM+inhibitor treatment compared to the Control. 100X, 1cm:100µm. WB (D) and RT-qPCR (E) results indicated that the DDM extract significantly upregulated RUNX2, BSP, Osx, COL I, OPN, OCN, OPG and RANKL. With the DDM+IDO1 inhibitor, RUNX2, BSP, Osx, COL I, OPN, OCN and OPG decreased significantly while RANKL remained unchanged. Neither DDM nor DDM+inhibitor affected RANK expression. CD4+BMSC, co-culture of naïve CD4 + T cells and BMSCs; DDM+BMSC, culture of BMSCs with the DDM extract; CD4+DDM+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract; CD4+DDM+Inh+BMSC, co-culture of naïve CD4 + T cells and BMSCs with the DDM extract and IDO1 inhibitor; DDM+Inh+BMSC, culture of BMSCs with the DDM extract and IDO1 inhibitor.

    Article Snippet: BMSCs were cultured in osteogenic induction media (Procell), and ALP staining was performed after 14 days.

    Techniques: Co-Culture Assay, CCK-8 Assay, Control, Staining, Quantitative RT-PCR, Expressing